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human exon 1.0st array  (Thermo Fisher)


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    Thermo Fisher human exon 1.0st array
    Human Exon 1.0st Array, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+array/nct04134260-8-18--1
    Average 90 stars, based on 1 article reviews
    human exon 1.0st array - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: Overexpression of Foxc1 regenerates crushed rat facial nerves by promoting Schwann cells migration via the Wnt/β‐catenin signaling pathway
    Article Snippet: The nerve tissues of the sham group and facial nerve injury (FNI) group were lysed immediately in 500 μl of TRIzol (Thermo Fisher Scientific, Waltham, MA) and were stored at −80°C before purification using a standard phenol‐chloroform extraction protocol with an RNAqueous Micro Kit (Thermo Fisher Scientific). .. The transcriptome was subjected to microarray analysis using an Affymetrix human array (Thermo Fisher Scientific) and normalized based upon quantiles. ..

    Article Title: Exosomal LncRNA–NEAT1 derived from MIF-treated mesenchymal stem cells protected against doxorubicin-induced cardiac senescence through sponging miR-221-3p
    Article Snippet: Microarray analysis Exosomes derived from MSCs, or MSCs treated with MIF, were lysed immediately in 500 μL of TRIzol reagent (ThermoFisher Scienti c, MA, USA) and stored at −80°C before puri cation using a standard phenol–chloroform extraction protocol with an RNAqueous Micro Kit (ThermoFisher Scienti c). .. The transcriptome was subjected to microarray analysis using an Affymetrix human array (ThermoFisher Scienti c) and normalized based on quantiles. ..

    Article Title: Co-culturing with hypoxia pre-conditioned mesenchymal stem cells as a new strategy for the prevention of irradiation-induced fibroblast-to-myofibroblast transition
    Article Snippet: A non-targeting siRNA was used as a negative control (Invitrogen; ThermoFisher Scientific, Inc.). .. The transcriptome was analyzed by microarray using an Affymetrix human array (Thermo Fisher Scientific, Inc.) and normalized based upon quantiles. ..

    Article Title: Exosomal LncRNA–NEAT1 derived from MIF-treated mesenchymal stem cells protected against doxorubicin-induced cardiac senescence through sponging miR-221-3p
    Article Snippet: Page 10/30 Microarray analysis Exosomes derived from MSCs, or MSCs treated with MIF, were lysed immediately in 500 μL of TRIzol reagent (ThermoFisher Scienti c, MA, USA) and stored at −80°C before puri cation using a standard phenol–chloroform extraction protocol with an RNAqueous Micro Kit (ThermoFisher Scienti c). .. The transcriptome was subjected to microarray analysis using an Affymetrix human array (ThermoFisher Scienti c) and normalized based on quantiles. ..

    Article Title: LncRNA-NEAT1 from the competing endogenous RNA network promotes cardioprotective efficacy of mesenchymal stem cell-derived exosomes induced by macrophage migration inhibitory factor via the miR-142-3p/FOXO1 signaling pathway
    Article Snippet: Exosomes and cardiomyocytes were lysed immediately in 500 μL TRIzol (ThermoFisher Scientific, Waltham, MA, USA) and stored at − 80 °C before purification using a standard phenol–chloroform extraction protocol with an RNAqueous Micro Kit (ThermoFisher Scientific). .. The transcriptome was subjected to microarray analysis using an Affymetrix human array (ThermoFisher Scientific) and normalized based upon quantiles. .. Total RNA was isolated from exosomes and cells using TRIzol (Ambion; ThermoFisher Scientific). cDNA was synthesized from 1 μg of total RNA using Superscript II reverse transcriptase, according to the manufacturer’s protocol.

    Genome Wide:

    Article Title: Denisovan, modern human and mouse TNFAIP3 alleles tune A20 phosphorylation and immunity.
    Article Snippet: 1Garvan Institute of Medical Research, Darlinghurst, New South Wales, Australia.. 2Faculty of Medicine, University of New South Wales, Sydney, New South Wales, Australia.. 3Department of Ophthalmology, Flinders University, Bedford Park, South Australia, Australia.

    Article Title: Mutations in FKBP10 can cause a severe form of isolated Osteogenesis imperfecta
    Article Snippet: .. Genomic DNA samples of family members I1-2, II1-2, and II4-5 were genotyped using the Affymetrix AxiomTM Genome-Wide Human Array, featuring 567,096 SNP markers (Affymetrix, Santa Clara, CA, USA). ..



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    Expected cellular responses of human articular chondrocytes under dynamic culture and IL‑1β‐induced inflammatory conditions. Representative results illustrate chondrocyte morphology, viability, extracellular matrix (ECM) expression, metabolic activity, and inflammatory responses under gravity‐driven flow conditions. (A) Representative z‐projection fluorescence images of chondrocytes cultured for 21 days in microchannels coated with a fibronectin–collagen type IV mixture. Cells form aggregates and microtissue‐like structures across regions I–III corresponding to increasing fluid shear stress levels. Cells are stained for F‐actin (red) and nuclei (DAPI, blue). (B) Representative Live/Dead fluorescence images showing time‐dependent viability and proliferation of chondrocytes cultured under dynamic conditions on days 3, 7, 14, and 21 (control), compared with IL‑1β‐stimulated cultures on day 21 (1 and 10 ng/ml, applied from day 15 to day 21). Control cultures show progressive formation of compact microtissue‐like aggregates (white arrows), whereas IL‑1β stimulation results in disrupted aggregates (yellow arrows). Green: calcein‐AM (live cells); red: ethidium homodimer‐1 (dead cells). (C) Representative z‐projection immunofluorescence images of ECM marker expression in region I after 21 days of culture, comparing control and IL‑1β‐stimulated samples. Images show merged fluorescence channels for collagen type II (green), aggrecan (red), and nuclei (DAPI, blue). (D–E) Quantification of corrected total cell fluorescence (CTCF) for collagen type II (D) and aggrecan (E) across regions I–III for control and IL‑1β‐stimulated cultures at day 21 (n = 44 cells per group). (F) Cell metabolic activity measured using a PrestoBlue assay for control and IL‑1β‐stimulated samples at day 21 ( n = 5 independent samples per group). (G–H) Quantification of inflammatory mediators in culture supernatant collected at day 21 using multiplex ELISA, including matrix metalloproteinases (MMP‐1, MMP‐2, MMP‐3, MMP‐7, MMP‐9, MMP‐13) and cytokines (IL‑1β, IL‑6, IL‑8) ( n = 3 independent samples per group). Values below the lower limit of quantification are indicated as <LLOQ. (I) Quantification of collagen type II fragments released into the culture medium at day 21, measured by ELISA ( n = 3 independent samples per group). All fluorescence images include a 100 µm scale bar, and white dashed lines indicate microchannel boundaries. Statistical analysis was performed using the Kruskal–Wallis test with Dunn's post hoc comparison (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; n.s., not significant). Conditions include control (no cytokine) and IL‑1β stimulation (1 and 10 ng/ml). Note: Panels are adapted from da Silva et al. , licensed under CC BY 3.0.

    Journal: Current Protocols

    Article Title: A Gravity‐driven Microfluidic Platform for High‐throughput Culture and Inflammatory Stimulation of Human Articular Chondrocytes

    doi: 10.1002/cpz1.70489

    Figure Lengend Snippet: Expected cellular responses of human articular chondrocytes under dynamic culture and IL‑1β‐induced inflammatory conditions. Representative results illustrate chondrocyte morphology, viability, extracellular matrix (ECM) expression, metabolic activity, and inflammatory responses under gravity‐driven flow conditions. (A) Representative z‐projection fluorescence images of chondrocytes cultured for 21 days in microchannels coated with a fibronectin–collagen type IV mixture. Cells form aggregates and microtissue‐like structures across regions I–III corresponding to increasing fluid shear stress levels. Cells are stained for F‐actin (red) and nuclei (DAPI, blue). (B) Representative Live/Dead fluorescence images showing time‐dependent viability and proliferation of chondrocytes cultured under dynamic conditions on days 3, 7, 14, and 21 (control), compared with IL‑1β‐stimulated cultures on day 21 (1 and 10 ng/ml, applied from day 15 to day 21). Control cultures show progressive formation of compact microtissue‐like aggregates (white arrows), whereas IL‑1β stimulation results in disrupted aggregates (yellow arrows). Green: calcein‐AM (live cells); red: ethidium homodimer‐1 (dead cells). (C) Representative z‐projection immunofluorescence images of ECM marker expression in region I after 21 days of culture, comparing control and IL‑1β‐stimulated samples. Images show merged fluorescence channels for collagen type II (green), aggrecan (red), and nuclei (DAPI, blue). (D–E) Quantification of corrected total cell fluorescence (CTCF) for collagen type II (D) and aggrecan (E) across regions I–III for control and IL‑1β‐stimulated cultures at day 21 (n = 44 cells per group). (F) Cell metabolic activity measured using a PrestoBlue assay for control and IL‑1β‐stimulated samples at day 21 ( n = 5 independent samples per group). (G–H) Quantification of inflammatory mediators in culture supernatant collected at day 21 using multiplex ELISA, including matrix metalloproteinases (MMP‐1, MMP‐2, MMP‐3, MMP‐7, MMP‐9, MMP‐13) and cytokines (IL‑1β, IL‑6, IL‑8) ( n = 3 independent samples per group). Values below the lower limit of quantification are indicated as

    Article Snippet: Chondrocyte growth medium (HC Growth Medium, Cell Applications, cat. no. 411‐500) DPBS (sterile, Gibco, cat. no. 14190144) Recombinant IL‐1β (R&D Systems, cat. no. 201‐LB‐025) (separately at −20°C) Live/Dead Viability/Cytotoxicity Kit containing calcein‐AM and ethidium homodimer 1 (EthD‐1) (Invitrogen, cat. no. LS224) PrestoBlue Cell Viability Reagent (Invitrogen, cat. no. A13261) 4% paraformaldehyde (PFA) in DPBS (Thermo Scientific, cat. no. J61899.AP) Normal donkey serum Bovine serum albumin (BSA) Triton X‐100 Tetramethylrhodamine (TRITC)‐phalloidin staining (EMD Millipore/Merck, cat. no. 90228) Mouse anti‐Collagen II antibody (NB600‐844, Novus Biologicals) Goat anti‐Aggrecan antibody (AF1220, R&D Systems) Rabbit anti‐Mouse IgG (H+L), FITC‐conjugated secondary antibody (NB7543, Novus Biologicals) Donkey anti‐Goat IgG (H+L), Northern Lights 557 (NL557)‐conjugated secondary antibody (NL001, R&D Systems) 4′,6‐diamidino‐2‐phenylindole (DAPI) Solution (Novus Biologicals, Bio‐Techne, cat. no. NBP2‐31156) Q‐Plex Human MMP 6 Plex kit (340949HU, Quansys Biosciences) Q‐Plex Human Custom Cytokine Plex kit (107749GR, Quansys Biosciences) Human collagen type II alpha 1 ELISA kit (BSKH63293, Bioss Antibodies) Pre‐established chondrocyte cultures generated using Basic Protocol Rocker platform (AKITA rocker, FinnAdvance or equivalent) CO incubator maintained at 37°C and 5% CO Class II biosafety cabinet Wide‐field fluorescence microscope (e.g., Leica DM4B or equivalent) equipped with blue/green and red/green excitation/emission filter sets.

    Techniques: Morphology, Expressing, Activity Assay, Fluorescence, Cell Culture, Shear, Staining, Control, Immunofluorescence, Marker, Prestoblue Assay, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Analysis, Comparison